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目的探讨miRNA-205在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)患者组织中的表达情况。方法收集56例经病理确诊的ESCC患者的手术切除组织(癌组织及其癌旁组织),采用实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qRT-PCR)检测miRNA-205的表达,并分析miRNA-205在不同临床病理资料之间的差异。结果 ESCC患者组织中miRNA-205相对表达量为1.475(0.444~5.478),显著高于癌旁组织(Z=-3.067,P=0.002);miRNA-205在不同TNM分期ESCC患者组织中存在差异,TNM分期越高,miRNA-205表达量越高,差异具有统计学意义(F=36.335,P<0.001);miRNA-205在淋巴结转移阴性/阳性组织中存在差异,淋巴结转移阳性组miRNA-205表达量显著高于淋巴结转移阴性组(Z=-3.353,P=0.001)。结论 miRNA-205在ESCC患者癌组织中表达升高,并可能参与ESCC的发生与发展。
Objective To investigate the expression of miRNA-205 in esophageal squamous cell carcinoma (ESCC) patients. Methods 56 cases of pathologically confirmed ESCC patients (cancer tissues and adjacent tissues) were collected and the miRNA-205 was detected by real-time quantitative polymerase chain reaction (qRT-PCR) The expression of miRNA-205 in different clinicopathological data was analyzed. Results The relative expression of miRNA-205 in ESCC patients was 1.475 (0.444-5.478), significantly higher than that in paracancerous tissues (Z = -3.067, P = 0.002). There was a difference in miRNA- The higher the TNM stage, the higher the expression of miRNA-205, the difference was statistically significant (F = 36.335, P <0.001). The difference of miRNA-205 in lymph node metastasis negative / positive tissues, lymph node metastasis positive group miRNA-205 expression Significantly higher than the negative lymph node metastasis group (Z = -3.353, P = 0.001). Conclusion The expression of miRNA-205 is increased in ESCC patients and may be involved in the occurrence and development of ESCC.