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目的探讨人乳头瘤病毒（ＨＰＶ）感染在宫颈癌变过程中的作用。方法应用聚合酶链反应（ＰＣＲ）和流式细胞计量术（ＦＣＭ）对３９例宫颈癌标本中ＨＰＶ１６和１８型感染、ＤＮＡ含量和细胞周期分布进行检测和分析。结果３１例鳞癌中ＨＰＶ１６阳性１１例（３５．５％），ＨＰＶ１８阳性１例；７例腺癌中ＨＰＶ１６全部阴性，ＨＰＶ１８阳性２例（２８．６％）；１例腺鳞癌ＨＰＶ１６和１８均阴性。总的异倍体率为５９％，二倍体率为４１％。鳞癌异倍体率为７１％（２０／３１），腺癌为４３％（３／７）。鳞癌中ＨＰＶ１６阳性组与阴性组间ＤＮＡ指数和Ｓ期比率有明显差异，而异倍体率无差异。结论ＨＰＶ感染与宫颈癌发生有关。
Objective To investigate the role of human papillomavirus (HPV) infection in cervical carcinogenesis. Methods The HPV16 and 18 infection, DNA content and cell cycle distribution in 39 cases of cervical cancer were detected by polymerase chain reaction (PCR) and flow cytometry (FCM). Results The positive rate of HPV16 was 11 (35.5%) in 31 squamous cell carcinomas and 1 was HPV18 positive. The negative rate of HPV16 in all 7 adenocarcinomas was HPV16 positive (28.6%). One case of adenosquamous carcinoma HPV16 and 18 All negative. The total aneuploidy rate was 59% and the diploid rate was 41%. Squamous cell aneuploidy rate was 71% (20/31), adenocarcinoma was 43% (3/7). In squamous cell carcinoma, the DNA index and S-phase ratio of HPV16-positive group and negative group were significantly different, while the aneuploidy rate was not different. Conclusion HPV infection is related to the occurrence of cervical cancer.