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本文选用了四种离子交换树脂进行分离纯化玻璃酸的研究，结果表明，采用 Ｄ３１５大孔离子交换树脂，以去离子水为淋洗液， ０．６ｍｏｌ／Ｌ ＮａＣｌ溶液为洗脱液，纯化效果较佳。纯化产品杂蛋白含量≤０．３％，回收率达７１．１％，粘均分子量大于９６万。
In this paper, four kinds of ion exchange resins were used to separate and purify hyaluronic acid. The results showed that the deionized water was used as eluent and 0.6mol / L NaCl solution was used as eluent to purify the hyaluronic acid with D315 macroporous ion exchange resin Better. Purified protein impurity content ≤ 0.3%, the recovery rate of 71.1%, viscosity average molecular weight of more than 960,000.