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目的观察中药复方扶正解毒含药血清(FJD)对硫酸镍(NiSO4)诱导培养人支气管上皮(16HBE)细胞恶性转化的抑制作用。方法NiSO4多次处理16HBE细胞,利用刀豆凝集素A(ConA)凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定;同时添加不同剂量的FJD,观察其抗转化效应。结果NiSO4(400μmol/L)染毒8次(20代)时,细胞生长速度加快,排列紊乱,失去接触抑制,出现复层生长,可被ConA凝集,并可在软琼脂上生长。3种剂量FJD与NiSO4共处理组细胞转化率分别为0.82%、0.47%和0.19%,明显低于单用NiSO4组(4.13%),且呈剂量-效应关系(r=-0.884,P<0.01),细胞ConA凝集反应阴性,不能在软琼脂上生长。结论体外实验显示,FJD具有抑制NiSO4诱导16HBE细胞恶性转化的作用。
Objective To observe the inhibitory effect of traditional Chinese medicine Fuzhengjiedu drug-containing serum (FJD) on the malignant transformation of human bronchial epithelial (16HBE) cells induced by nickel sulfate (NiSO4). Methods NiSO4 was used to treat 16HBE cells multiple times. Concan agglutinin A (ConA) agglutination assay and soft agar colony formation assay were used to identify the transformed cells. Different doses of FJD were added to observe the anti-transformation effect. Results When NiSO4 (400μmol/L) was exposed for 8 times (20 passages), the growth rate of the cells was increased, disordered, loss of contact inhibition, multiple layer growth, agglutination by ConA, and growth on soft agar. The cell transformation rates of the three doses of FJD and NiSO4 co-treatment group were 0.82%, 0.47% and 0.19%, respectively, which was significantly lower than that of the NiSO4 alone group (4.13%) and showed a dose-effect relationship (r=-0.884, P<0.01). ), Cell ConA agglutination was negative and could not grow on soft agar. Conclusion In vitro experiments showed that FJD could inhibit the malignant transformation of 16HBE cells induced by NiSO4.