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[Objective] To provide axenic explants for tissue culture of Oenothera biennis L..[Method] Oenothera biennis L.seeds were inoculated into different culture mediums after being sterilized with different methods,the contamination rate and germination rate were calculated 10 days later.[Result] After being treated for 48 h at 70 ℃,the degerming effect of ethanol(40 s) + 0.1% Hg2Cl2(10 min)+ Tween 80 on the surface of explants was the best;the germination rate of Oenothera biennis L.seed reached 93% in VW+1.0 mg/L 6-BA+0.5 mg/L NAA+0.05% AC culture medium and the growth condition of seedling was the best.[Conclusion] The good and effective aseptic germination system was established in this study,which would provide basis for the persistent utilization of Oenothera biennis L..
[Objective] To provide axenic explants for tissue culture of Oenothera biennis L .. [Method] Oenothera biennis L.seeds were inoculated into different culture mediums after being sterilized with different methods, the contamination rate and germination rate were calculated 10 days later. [ Result] After being treated for 48 h at 70 ° C, the degerming effect of ethanol (40 s) + 0.1% Hg2Cl2 (10 min) + Tween 80 on the surface of explants was the best; the germination rate of Oenothera biennis L. seed reached 93% in VW + 1.0 mg / L 6-BA + 0.5 mg / L NAA + 0.05% AC culture medium and the growth condition of seedling was the best. [Conclusion] The good and effective aseptic germination system was established in this study , which would provide basis for the persistent utilization of Oenothera biennis L.