【摘 要】
：
The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years,though no GAPs have been explored in epididy
【机 构】
：
College of Life Science, Sichuan University, Chengdu 610064, China;Shanghai Key Laboratory for Molec
论文部分内容阅读
The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years,though no GAPs have been explored in epididymis, an essential organ for sperm maturation. In this study, a new GAP member, designated as MacGAP, was cloned in human epididymis. The MacGAP gene encodes a protein of 618 amino acids with a putative size of 70 kDa and harbors the conservedRhoGAPdomain. The N-terminal and C-terminal peptides of MacGAP were expressed and their corresponding antiserawere prepared. The antisera against N-terminal peptide could detect antigen as low as 0.3 ng, and its specificity was alsoconfirmed. However, the antisera against C-terminal peptide failed to detect its antigen because of its low sensitivity.Immunohistochemistry showed that the MacGAP protein was dependent on epididymis and had a region-specific expression patt, with high expression in the epithelial cells’ basal section in the caput region. The results have created a foundation for further interpretation of the biological effects of GAPs in sperm maturation.
其他文献
The experimental infertility model of treatments involving testicular warming, testosterone implant, and a combination of the two was developed to confirm a syn
Huntingtin (Htt) mutation causes Huntington’s disease. Sequence analysis of Htt revealed a possible thrombin cleavage site in the N-terminal region of Htt. In
Macrophage death in advanced atherosclerotic lesions leads to iesional necrosis, possible plaque rupture, and acute vascular occlusion. A likely cause of macrop
Tyrosine kinase with immunoglobulin and epidermal growth factor homology domain-2 (Tie2) has been considered as a rational target for gene therapy in solid tumo
本研究以26个苹果品种和杂种个体为试材，对其果实的营养品质、加工品质进行比较，对其所酿的苹果醋进行理化指标分析和感官评价，筛选出适宜酿醋的苹果品种（系）。并对所酿苹果醋的香
As the first identified scorpion toxin active on both big conductance Ca2+-activated K+ channels (BK) and small conductance Ca2+-activated K+ channels (SK), BmB
Oithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis that decarboxylates oithine to putrescine, has become a promising target for can
新油（杂）６号、７号的杂交优势刘发海，尹经章（新疆农业大学，乌鲁木齐，８３００００）新油（杂）６号、７号是新疆农业大学农学分院１９８７年引进三系，经育性鉴定选配组合，选育而成的杂交新品种。１９９４年６月新缰农作物品种审定
为了满足棉农对良种的需要,太仓县从1985年起在良种繁育工作中实行三个改革,即繁育方法改革、收购保贮改革、供种办法改革。1、种子繁育。改过去由县每年大量选
In order t