背景 采用体外培养的AC133 CD34~+细胞快速扩增cDNA5’末端技术,最近发现转录起始点在以前确定的转录起始点上游大约0.7 kb。在红细胞系统和上皮系统中可以证实转录起始于潜换外显子1a。因为外显子1a核酸序列不包含ATG密码子,作者检查外显子1a的转录起始点是否会导致功能性糖基转移酶的产生。研究设计和方法 采用多种表达A转移酶的质粒稳定转染人胃癌MKN28细胞。结果 大量A抗原被证实表 Background The rapid amplification of cDNA 5 ’end technology using AC133 CD34 + cells cultured in vitro recently found that the transcription start point was approximately 0.7 kb upstream of the previously identified transcription initiation point. In the erythrocyte system and the epithelial system, transcription initiation from the transitional exon 1a can be confirmed. Because the exon 1a nucleic acid sequence does not contain ATG codons, the authors examined whether the transcriptional start of exon 1a would result in the production of a functional glycosyltransferase. Study Design and Methods Stable transfected human gastric cancer MKN28 cells using various plasmids expressing A transferase. Results A large number of antigens were confirmed