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目的探讨螺旋藻(SP)对四氧化二氮导致的动物损伤的防护作用。方法采用在静式染毒柜内吸入N2O4方式构建动物损伤模型,染毒柜体积120L,柜内温度保持在(25.0±0.5)℃,染毒柜内N2O4注入剂量为45μl,每隔5min向柜内补充N2O41μl,吸入染毒时间为30 min。ICR雄性小鼠随机分为正常对照,N2O4单纯染毒,SP低浓度+染毒,SP中浓度+染毒和SP高浓度+染毒,共5组,每组15只。SP分别以0.5、1.0、1.5g/kg配成灌喂液,提前4d经口灌胃,每只灌喂0.4 ml。第5日灌胃后1h进行染毒,前后共灌胃5d。脱臼法处死后取材,测定肺组织SOD、GSH-Px活性和MDA含量;取肺组织切片观察病理变化。结果 1.0g/kg SP分别提高SOD 38.9%,GSH-Px 73.9%,MDA 83.0%。肺组织病理观察显示:SP组较单纯染毒组病变轻。结论 SP对四氧化二氮导致的动物损伤有显著的防护作用。
Objective To investigate the protective effect of Spirulina (SP) on animal injury caused by nitrous oxide. Methods The model of animal injury was established by inhalation of N2O4 in a static toxin cabinet. The volume of the exposed cabinet was 120L, the temperature inside the cabinet was maintained at (25.0 ± 0.5) ℃. The dosage of N2O4 in the toxin cabinet was 45μl. Add N2O41μl, inhalation exposure time is 30 min. ICR male mice were randomly divided into normal control, N2O4 single exposure, SP low concentration + exposure, SP concentration + exposure and SP exposure + concentration. SP were 0.5,1.0,1.5 g / kg dosing solution, 4d early oral gavage, each fed 0.4 ml. On the 5th day, the rats were treated with 1 hour after intragastric administration, and were given intragastrically for 5 days. After dislocation method, the rats were sacrificed and the activity of SOD, GSH-Px and the content of MDA in the lung tissue were measured. The lung tissue sections were taken to observe the pathological changes. Results 1.0g / kg SP increased SOD 38.9%, GSH-Px 73.9%, MDA 83.0%. Pathological observation of lung tissue showed that the lesions in SP group were lighter than that in purely infected group. Conclusion SP has a significant protective effect on animal injury caused by nitrous oxide.