论文部分内容阅读
实验发现在十二烷基苯磺酸钠(SDBS)存在下,利多卡因能增强血清白蛋白的共振光散射强度,据此,建立了以利多卡因为探针,利用共振光散射法测定牛血清白蛋白(BSA)和人血清白蛋白(HSA)含量的新方法。考察了反应时间、试剂的加入顺序、pH值、SDBS和利多卡因的浓度以及共存干扰物等因素对共振光强度的影响。在优化的条件下,测定BSA和HSA的线性范围分别为1.0～45.0和0.5～30.0 mg.L-1。该方法用于人血清样品中蛋白含量的分析,获得了较高的精密度和准确度,五次平行测定的相对标准偏差在4.9%～5.7%之间,加标回收率在90%～103%之间。该方法使用常规荧光仪和常用化学试剂即可测定,操作简便、具有较高的灵敏度(检出限为0.14 mg.L-1)。对新鲜的人血清样品可以直接进行分析,无需进行样品前处理。本方法为人血清样品中蛋白含量的测定提供了一个可供选择的新途径。
It was found that lidocaine enhanced the intensity of serum albumin resonance light scattering in the presence of sodium dodecylbenzenesulfonate (SDBS). Based on this, lidocaine was used as a probe to determine the concentration of bovine serum albumin Serum albumin (BSA) and human serum albumin (HSA) content of the new method. The effects of reaction time, order of adding reagents, pH value, concentrations of SDBS and lidocaine, and coexisting interferers on the resonance light intensity were investigated. Under the optimized conditions, the linear range of BSA and HSA was 1.0 ~ 45.0 and 0.5 ~ 30.0 mg.L-1, respectively. The method was applied to the analysis of protein content in human serum samples with high precision and accuracy. The relative standard deviations of the five parallel determinations were between 4.9% and 5.7%, and the recoveries of spiked samples ranged from 90% to 103 %between. The method can be determined using a conventional fluorometer and commonly used chemical reagents. It is simple to operate and has high sensitivity (limit of detection 0.14 mg.L-1). Fresh human serum samples can be analyzed directly without the need for sample preparation. The method provides a new alternative for the determination of protein content in human serum samples.