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目的和方法：采用间隔２４ｈ两次注射大肠杆菌内毒素（ＥＴ）的方法，复制家兔内毒素性急性肺损伤模型，探讨肺损伤的机理。结果：ＥＴ组血浆、肺组织匀浆及支气肺泡灌洗液（ＢＡＬＦ）中白细胞介素８（ＩＬ－８）、亚硝酸／硝酸根离子（ＮＯ２－／ＮＯ３－）水平显著增高（Ｐ＜００１），血浆补体Ｃ５ａ活性明显增高（Ｐ＜００１），ＢＡＬＦ内中性粒细胞明显增多，肺系数、肺水含量及通透指数升高。ＩＬ－８、ＮＯ２－／ＮＯ３－水平以及血清和ＢＡＬＦ中酸性磷酸酶活性变化高度相关。结论：ＩＬ－８、一氧化氮（ＮＯ）参与内毒素性急性肺损伤，而ＩＬ－８是造成肺损伤的重要中间环节。
PURPOSE AND METHODS: The model of acute lung injury induced by endotoxin in rabbits was replicated by injecting E. coli endotoxin twice (ET) 24 hours apart to investigate the mechanism of lung injury. Results: The levels of IL-8 and nitrite / nitrate (NO2- / NO3-) in plasma, lung homogenates and BALF of ET group were significantly increased (P < 0 01), plasma C5a activity was significantly increased (P <0 01), BALF neutrophils increased significantly, lung coefficient, lung water content and permeability index increased. IL-8, NO2- / NO3- levels and changes in acid phosphatase activity in serum and BALF. CONCLUSION: IL-8 and NO are involved in endotoxin-induced acute lung injury, while IL-8 is an important intermediate link in lung injury.