论文部分内容阅读
目的探讨声音信号强度对豚鼠下丘给声响应神经元间隔探测的影响。方法采用单细胞记录的方法,刺激信号由100 ms和50 ms白噪声相加组成,声音强度10～80 dB SPL,中间间隔时间为0、1、2、4、8、16、48、96 ms,分别统计阈响应组和阈上10,20,30 dB时,给声响应神经元的间隔阈值,并观察间隔阈值与放电率和潜伏期的关系。结果本实验共记录96个神经元,其中,给声响应神经元有35个,在阈响应强度时,给声响应神经元间隔阈值明显增大,四组强度的间隔阈值均值分别为(22.91±4.36)ms,(9.00±2.69)ms,(4.00±0.49)ms和(11.33±3.11)ms(P<0.01),但阈上各组间的差异无统计学意义(P>0.05)。在间隔时间为48 ms时,给声响应神经元间隔后放电恢复率的均值分别为0.44±0.15,0.83±0.12,0.88±0.07和0.61±0.10(P<0.01)。而首次放电潜伏期分别为(12.86±0.72)ms,(11.65±0.64)ms,(11.03±0.65)ms和(10.68±0.55)ms(P>0.05)。结论声音信号强度对豚鼠下丘给声响应神经元的间隔探测有一定影响。
Objective To investigate the influence of sound signal intensity on the detection of acoustic response neurons in inferior colliculus of guinea pigs. Methods The single cell recording method was used. The stimulus signal consisted of white noise of 100 ms and 50 ms. The intensity of sound was 10 ~ 80 dB SPL with the interval of 0,1,2,4,8,16,48 and 96 ms , Respectively, the threshold response group and the threshold above 10,20,30 dB, to the acoustic response neurons interval threshold, and to observe the interval threshold and the discharge rate and latency. Results A total of 96 neurons were recorded in this experiment. Among them, 35 neurons were given acoustic response. At the threshold response intensity, the threshold of neuron interval was significantly increased, and the mean interval thresholds of the four groups were (22.91 ± 4.36 ms, (9.00 ± 2.69) ms, (4.00 ± 0.49) ms and (11.33 ± 3.11) ms respectively (P <0.01), but there was no significant difference between the three groups (P> 0.05). When the interval time was 48 ms, the mean value of discharge recovery after giving neurons was 0.44 ± 0.15, 0.83 ± 0.12, 0.88 ± 0.07 and 0.61 ± 0.10, respectively (P <0.01). The first discharge latency was (12.86 ± 0.72) ms, (11.65 ± 0.64) ms, (11.03 ± 0.65) ms and (10.68 ± 0.55) ms respectively (P> 0.05). Conclusion The intensity of sound signal has some effect on the interval detection of the acoustic response neurons in the inferior colliculus of guinea pigs.