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目的:研究Z,E-butylidedephthalide (Bdph)对激光诱导的大鼠脉络膜新生血管(CNV)和兔眼脉络膜血流的影响。方法:雄性Brown Norway大鼠行Nd:YAG激光诱导眼底Bruch膜破裂,而后分别予30mg/kg和15mg/kgBdph1次/d腹腔注射,连续4wk。2wk及4wk末行眼底荧光血管造影检查。4wk末制作脉络膜平片测量新生血管的面积。用1g/LZ,E-butylidenephthalide给雌性新西兰大白兔点眼,采用彩色微球技术测量大白兔眼部血流的变化。结果:和对照组相比,Bdph30mg/kg和15mg/kg组大鼠眼底血管荧光渗漏明显减少,P<0.01;在此两组中,眼底荧光血管造影测定的新生血管面积以及脉络膜平片测定的新生血管面积都比对照组减少。10g/LZ,E-butylid-eneph-thalide滴入兔眼30和60min后脉络膜血流均比对照组增加,P<0.05。结论:Z,E-butylidedephthalide可以抑制大鼠脉络膜新生血管的生长,增强兔眼脉络膜血流,可能成为治疗年龄相关性黄斑变性的新药。
Objective: To investigate the effects of Z, E-butylidedephthalide (Bdph) on choroidal neovascularization (CNV) and choroidal blood flow in rabbit eyes. Methods: Male Brown Norway rats underwent Nd: YAG laser-induced Bruch’s membrane rupture, and then injected intraperitoneally with 30 mg / kg and 15 mg / kg Bdph1 once daily for 4 weeks. 2wk and 4wk end fundus fluorescein angiography. 4wk at the end of the production of choroidal flat film neovascularization area. Female New Zealand white rabbits were spotted with 1 g / L Z, E-butylidenephthalide, and the changes of ocular blood flow in rabbits were measured by color microspheres technique. Results: Compared with the control group, the fluorescein leakage of fundus in Bdph 30 mg / kg and 15 mg / kg groups decreased significantly (P <0.01). In both groups, the area of neovasculature determined by fundus fluorescein angiography and choroidal plaque determination Of the neovascularization area than the control group decreased. The choroidal blood flow of 10g / LZ and E-butylid-eneph-thalide dropped into the rabbit eyes 30 and 60 minutes later than that of the control group, P <0.05. Conclusion: Z, E-butylidedephthalide can inhibit the growth of rat choroidal neovascularization and enhance the choroidal blood flow in rabbit eyes, which may become a new drug for the treatment of age-related macular degeneration.