Microencapsulated hepatocytes and islets as in vivo bioartificial liver support system

来源 :World Journal of Gastroenterology | 被引量 : 0次 | 上传用户:javapages
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AIM:To confirm the xenotransplantation of microencapsulatedhepatocytes and islets as a temporary bioartificial liversupport system for mice with acute liver failure (ALF).METHODS:Mice were rendered ALF by a single intra-peritoneal injection of D-galactosamine (D-gal) and theirtail blood was sampled to examine differences in blood ALT,albumin (ALB),total bilirubin (TB) and glucose (GLU) between4 experimental groups.Rat hepatocytes and islets werecollected and microencapsulated referring to both Sun’s andFritschy’s methods.Mice were grouped into control group(CG),free hepatocyte group (FHG),microencapsulatedhepatoo/te group (MHG) and microencapsulated hepatocyteplus islet group (HIG).Tissue samples were subjected tomicroscopic and electron microscopic (EM) examinations.RESULTS:The highest survival was observed in HIG,surprisingly at 100%(16/16),while the lowest was in CG at12.5%(2/16),with inter-group statistical difference P<0.05.ALT levels revealed no statistical difference between groupsbut the ALB level of HIG descended by the slightest margin{q=(0.54,0.24,1.33),P<0.05} at the time when it reachedthe lowest point in all groups.TB of HIG returned to normalreference range (NRR) statistically sooner than that of othersafter a fierce elevation.No statistical inter-group differencewas observed in GLU levels.Fusion between hepatocytesand beta cells was demonstrated giving rise to theoreticalassumptions.CONCLUSION:Hepatocytes to be microencapsulatedtogether with islets should be a preferred in viyo hepaticfunctional supporting system,which can dramatically prolongsurvival and improve living status. AIM: To confirm the xenotransplantation of microencapsulated hepatocytes and islets as a temporary bioartificial liversupport system for mice with acute liver failure (ALF). METHODS: Mice were rendered ALF by a single intra-peritoneal injection of D-galactosamine (D-gal) blood was sampled to examine differences in blood ALT, albumin (ALB), total bilirubin (TB) and glucose (GLU) between4 experimental groups. Rat hepatocytes and islets were collected and microencapsulated referring to both Sun’s and Fritschy’s methods. CG), free hepatocyte group (FHG), microencapsulated hepate / te group (MHG) and microencapsulated hepatocyte plus islet group (HIG). Tissue samples were subjected tomicroscopic and electron microscopic (EM) examinations.RESULTS: The highest survival was observed in HIG, surprisingly while the lowest was CG at 12.5% ​​(2/16), with inter-group statistical difference P <0.05.ALT levels revealed no statistical difference between gro upsbut the ALB level of HIG descended by the slightest margin {q = (0.54,0.24,1.33), P <0.05} at the time when it reached the lowest point in all groups. TB of HIG returned to normalreference range (NRR) than that of others after a fierce elevation. No statistical inter-group difference was observed in GLU levels. Fusion between hepatocytes and beta cells was demonstrated giving rise to theoreticalassumptions. CONCLUSION: Hepatocytes to be microencapsulated together with islets should be a preferred in viyo hepaticfunctional supporting system, which can enorm prolongsurvival and improve living status.
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