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目的:研究活性维生素D3对大鼠系膜增生性肾小球肾炎的作用及机制。方法:应用抗大鼠胸腺细胞免疫血清(Anti-thymo-cyte serum,ATS)抗体一次性尾静脉注射复制大鼠系膜增生性肾小球肾炎(Mesangial Proliferative Glomerulonephritis,MsPGN)模型,将实验大鼠分为三组对照组(A组)、模型组(B组)、活性维生素D3治疗组(C组),分别与1、3、7、14天每组各处死6只大鼠,并采用Envision免疫组化法、RT-PCR法,观察PCNA蛋白、PCNAmRNA及中性粒细胞在肾小球中的表达情况。结果:肾炎组增殖细胞核抗原(Proliferating cell nuclear Antigen,PCNA)蛋白、PCNAmRNA及肾小球中中性粒细胞计数明显高于对照组(p<0.01),并随着时间延长逐渐增高,到第7天达到高峰,第14天有下降的趋势。活性维生素D3治疗组PCNA蛋白、PCNAmRNA及肾小球中中性粒细胞计数的表达均较低(p<0.01),其中第3、7、14天表达均显著减少(p<0.05)。PCNA蛋白、PCNAmRNA、中性粒细胞计数各指标之间呈均成正相关。结论:活性维生素D3可以抑制系膜细胞的增生,其作用机制可能是抑制炎症细胞浸润、PCNAmRNA及其蛋白的表达。
Objective: To investigate the effect and mechanism of active vitamin D3 on mesangial proliferative glomerulonephritis in rats. Methods: A rat model of Mesangial Proliferative Glomerulonephritis (MsPGN) was induced by a single tail vein injection of Anti-thymo-cyte serum antibody (ATS) The rats in each group were divided into three groups: control group (group A), model group (group B) and active vitamin D3 treatment group (group C). Six rats were sacrificed on each of 1,3,7,14 days. Envision Immunohistochemistry and RT-PCR were used to observe the expression of PCNA protein, PCNA mRNA and neutrophils in the glomerulus. Results: Proliferating cell nuclear antigen (PCNA), PCNA mRNA and glomerular neutrophil count in nephritic group were significantly higher than those in control group (p <0.01), and gradually increased with time. By the seventh Days peaked, there is a downward trend on the 14th day. The expression of PCNA protein, PCNA mRNA and neutrophil count in glomeruli were lower in active vitamin D3 treatment group (p <0.01), and were significantly decreased on the 3rd, 7th and 14th day (p <0.05). PCNA protein, PCNA mRNA, neutrophil count showed a positive correlation between the various indicators. CONCLUSION: Active vitamin D3 can inhibit the proliferation of mesangial cells. Its mechanism may be to inhibit the infiltration of inflammatory cells and the expression of PCNA mRNA and protein.