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目的 :探讨人参皂甙Rg1对多巴胺诱导PC12细胞凋亡的保护作用及可能机制。 方法 :采用PC12细胞为模型 ,以不同浓度的DA来诱导细胞凋亡的发生 ,用流式细胞仪检测PC12细胞的亚二倍体峰 ,按Griess法测定NO代谢产物NO2 ·的浓度 ,并观察人参皂甙Rg1(10 μmol/L)预防性给药对其影响。 结果 :0、0 15、0 30、0 45和 0 6 0mmol/LDA处理组的凋亡率分别为 1 6 1±0 18、40 6 0± 1 74、46 6 7± 1 45、5 4 49± 1 6 1和 6 4 39± 1 6 0 % ,与Rg1预防组 0 73± 0 11、1 16± 0 0 9、1 35± 0 0 7、1 5 3± 0 10和 1 6 1± 0 12 %比较均有显著性差异 (P <0 .0 1) ;DA处理组的NO2 ·浓度分别为 1 82± 0 0 5、3 5 7± 0 0 2、8 82± 0 0 4、18 0 0± 0 0 7和 2 7 34± 0 0 9μmol/L ,与Rg1预防组的 1 0 0± 0 0 7、1 36± 0 0 7、1 2 5± 0 0 4、3 39± 0 11和 3 82± 0 11μmol/L比较亦都有显著性差异 (P <0 .0 1)。结论 :NO生成增多可能是DA诱导PC12细胞凋亡的重要信号分子 ,人参皂甙Rg1可抑制NO生成 ,防止DA诱导PC12细胞凋亡
Objective :To investigate the protective effect of ginsenoside Rg1 on apoptosis induced by dopamine in PC12 cells and its possible mechanism. METHODS: PC12 cells were used as a model to induce apoptosis at different concentrations of DA. The subdiploid peaks of PC12 cells were detected by flow cytometry. The concentration of NO metabolites NO2 · was measured by Griess method and observed. Effect of preventive administration of ginsenoside Rg1 (10 μmol/L) on it. Results The apoptotic rates of the 0, 0 15, 0 30, 0 45, and 0 6 0 mmol/LDA-treated groups were 1 6 1±0 18, 40 6 0 1 74, 46 6 7 1 45, 5 4 49 ± 1 6 1 and 6 4 39 ± 160%, with the Rg1 prevention group 0 73 ± 0 11, 1 16 ± 0 0 9, 1 35 ± 0 0 7, 1 5 3 ± 0 10, and 1 6 1 ± 0 There was a significant difference in 12% (P < 0.01); the NO2 concentration in the DA treated group was 1 82 ± 0 0 5, 3 5 7 ± 0 0 2, 8 82 ± 0 0 4, 18 0, respectively. 0 ± 0 0 7 and 2 7 34 ± 0 0 9 μmol/L, with 100 ± 0 07, 36 ± 0 07, 1 25 ± 0 4 and 3 39 ± 0 11 in the Rg1 prevention group. 3 82± 0 11μmol/L also showed significant differences (P < 0.01). Conclusion: Increased NO production may be an important signal molecule for DA-induced apoptosis of PC12 cells. Ginsenoside Rg1 can inhibit NO production and prevent DA from inducing apoptosis in PC12 cells.