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利用栓菌(Trametes sp.LS-10C)漆酶在单酶和漆酶-介体体系2种情况下对3种直接类偶氮染料进行脱色.结果表明,在给酶量为10 U·mL-1、染料浓度为100 mg· mL-1条件下反应24 h后,直接蓝86在非介体体系中脱色率达到71.2％,其最适脱色温度和pH范围分别为45 ～ 60℃和3.5 ～5.0;直接橙26在介体体系(10 μmol·L-1 ABTS)中脱色率达到78.7％,其最适脱色温度和pH范围分别为35 ～ 45℃和4.5 ～6.5;直接红31在介体体系(10 μmol·L-1ABTS)中脱色率达到90.7％,其最适脱色温度和pH范围分别为35 ～ 45℃和3.5～5.0.“,”Laccase from Trametes sp.LS-10C was used for decolorization of three kinds of direct azo dyes in both single enzyme (non-mediator) and enzyme-mediator systems.The results showed that for 10 U · mL-1 enzyme,the dye concentration was 100 mg · mL-1.After 24 h incubation,the decolorization rate of Direct Blue 86 in a non-mediator system reached 71.2％,with optimum decolorization temperature and pH of 45 to 60 ℃ and 3.5 to 5.0,respectively.Similarly,the decolorization rate of Direct Orange 26 in a mediator system (10 μmol · L-1 ABTS) reached 78.7％,with optimum decolorization temperature and pH of 35 to 45 ℃ and 4.5 to 6.5,respectively,and the decolorization rate of Direct Red 31 in a mediator system (10 μmol · L-1 ABTS) reached 90.7％,with optimum decolorization temperature and pH of 35 to 45 ℃ and 3.5 to 5.0,respectively.