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迄今,用于基因治疗的载体主要来自逆转录病毒中的鼠白血病病毒(MLV),但MLV载体仅在分裂期细胞中转导外源基因,限制了其在非分裂细胞如肝细胞、神经元等中的应用。人类免疫缺陷病毒(HIV)因蛋白MA和vpr能主动运输前整合复合物通过核小孔,有利于整合,故体外能感染非分裂的单核-巨噬细胞和T细胞。利用这一特性,作者构建了3种重组质粒:①包装重组体pCMV△R9,在HCMV即刻早期启动子启动下,表达HIV复制所需的全部反式激
To date, the vectors used for gene therapy are predominantly derived from murine leukemia virus (MLV) in retroviruses, but MLV vectors transduce foreign genes only in mitotic cells, limiting their role in non-dividing cells such as hepatocytes, neurons Etc. in the application. Human immunodeficiency virus (HIV) is capable of infecting non-dividing mononuclear macrophages and T cells in vitro due to the fact that proteins MA and vpr actively transport the integrative complex through the nuclei and facilitate integration. Using this feature, the authors constructed three recombinant plasmids: ① packaging recombinant pCMV △ R9, immediate promoter in HCMV immediate start, the expression of all HIV transfection required for transactivation