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将携带HBsAg基因的质粒DNA，直接注射到小鼠肌肉作为实验组；对照分成2组，一组注射携带β－半乳糖苷酶基因的质粒DNA，另一组注射商品化的血源性乙型肝炎疫苗、4周后从小鼠尾静脉采血，用ELISA方法检测血清中的抗－HBs。结果显示，实验组和注射乙型肝炎疫苗的对照组小鼠血清阳转率分别为5／6和4／6，而注射携带β－半乳糖苷酶的质粒DNA对照组小鼠血清全部为阴性。实验组小鼠血清中抗－HBs的水平与注射乙型肝炎疫苗的对照组小鼠类似，均在免疫后6～16周达到高峰，之后开始下降。抗体持续至少达半年以上。
The plasmid DNA carrying HBsAg gene was directly injected into mouse muscle as experimental group. The control group was divided into two groups, one group was injected with plasmid DNA carrying β-galactosidase gene and the other group was injected with commercial blood type B Hepatitis vaccine, blood was collected from the tail vein of mice 4 weeks later, and anti-HBs in serum was detected by ELISA. The results showed that the seroconversion rate of the mice in the control group and the control group vaccinated with hepatitis B vaccine were 5/6 and 4/6, respectively, while those in the plasmid DNA control group carrying β-galactosidase were all negative . The level of anti-HBs in the serum of experimental mice was similar to that of the control mice injected with Hepatitis B vaccine, and peaked at 6-16 weeks after immunization, then began to decrease. Antibodies persist for at least six months or more.