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为了了解蓝萼甲素在蓝萼香茶菜内的分布及不同生长期的消长情况,我们用高效液相色谱法对其不同部位及不同采集期的样品进行了蓝萼甲素的含量测定。 (1)仪器:岛津高效液相色谱仪LC-6A;岛津SPD-6AV紫外分光光度检测器;岛津C-R3A色谱数据处理机。色谱柱:岛津CLC-ODS C18十八烷基反相柱;柱压100kg/cm~2。流动相:甲醇∶水=60∶40;流
In order to understand the distribution of blue calyxin in the blue calyx fragrant tea cabbage and the growth and decline of different growth stages, we used high performance liquid chromatography in different parts of different samples and collected during the determination of blue calurril content. (1) Instruments: Shimadzu high performance liquid chromatography LC-6A; Shimadzu SPD-6AV UV spectrophotometric detector; Shimadzu C-R3A chromatographic data processor. Column: Shimadzu CLC-ODS C18 octadecyl reversed-phase column; column pressure 100kg / cm ~ 2. Mobile phase: methanol: water = 60:40; flow