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为高效集约经营云南乡土珍贵用材树种西南桦,在云南思茅、德宏选22个优良无性系为试材,从取材、材料的处理、外植体的诱导分化、芽的增殖、生根等方面探讨了西南桦组织培养繁殖技术和方法,结果表明,西南桦无性系间增殖系数及生根率均有一定差异,适宜增殖培养基为MS+6-BA 1.0 mg/L+KT 0.5 mg/L,增殖系数可达3~6倍,生根培养基为1/2 MS+IBA 1.0 mg/L+NAA 2.0 mg/L,生根率可达94.4%。
In order to efficiently and intensively manage the native trees of Betula platyphylla in Yunnan, 22 elite clones were chosen as test materials from Yunnan Simao and Dehong, and from the aspects of material drawing, material treatment, inducement and differentiation of explants, bud proliferation and rooting The results showed that the proliferation coefficient and rooting rate of Betula alnoides were different, and the optimal culture medium was MS + 6-BA 1.0 mg / L + KT 0.5 mg / L, and proliferation The coefficient of up to 3 ~ 6 times, rooting medium is 1/2 MS + IBA 1.0 mg / L + NAA 2.0 mg / L, the rooting rate of up to 94.4%.