论文部分内容阅读
通过中棉所 12 与 8891 的杂交及多代自交,获得由 180 个家系构成的重组自交系 F8、F9群体。重组自交系群体、两亲本及 F1于 2002、2003 两年种植; 对株型性状进行了研究,两年共调查了 10 个株型形状。利用该重组自交系群体,采用 SSR 为主体的分子标记构建了遗传连锁图,并对株型性状进行了单位点和双位点水平的 QTL 定位。结果表明,QTL 加性效应和上位性互作效应作为棉花重组自交系株型性状的遗传基础起着重要作用; 中棉所 12 与 8891 间多态性位点偏少,而表型差异较大且其杂交种湘杂棉二号有很强的杂种优势,QTL 互作可部分解释这一现象;结合对产量品质性状的研究结果,认为上位性可能是湘杂棉二号杂种优势的重要遗传基础。
Through the crosses of CIMC 12 and 8891 and selfing at many generations, F8 and F9 populations of recombinant inbred lines composed of 180 pedigrees were obtained. Recombinant inbred lines, parents and F1 were planted in two years in 2002 and 2003. Plant type traits were studied, and two plant types were investigated in two years. Using this recombinant inbred line population, SSR-based molecular markers were used to construct the genetic linkage map, and QTL mapping of plant type traits at single and double loci was conducted. The results showed that the QTL additive effect and epistasis interaction played an important role as the genetic basis of plant type traits in cotton inbred lines. The polymorphic loci of 12 and 8891 in Zhongmiandao were less, Large and its hybrid Xiangzamian 2 has a strong heterosis, QTL interaction can explain this phenomenon in part; combined with yield quality traits, the epistasis may be Xiangmou Mian two heterosis is important Genetic basis.