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对210例不同季节婴幼儿胃肠炎患儿的粪便,进行直接电镜、(DEM)、酶联免疫吸附试验(ELISA)、聚丙烯酰胺凝胶电泳(PAGE)、细菌培养和生化方法检查,并结合临床分析首次发现:昆明地区一年四季均有婴幼儿轮状病毒胃肠炎发生,病程可为急性、迁延性和慢性。全都排稀水便,25.4%伴有粘液便,部分病程迁延或伴粘液使者与混合感染有关。各季节婴儿轮状病毒胃肠炎的临床表现无差异(P>0.05),同时也发现不同电泳型轮状病毒胃肠炎的临床表现不同,提示不电泳型轮状病毒的毒性不同。尚指出了病毒与细茵、病毒之间混合感染的特点。
The stool samples of 210 infants and young children with gastroenteritis in different seasons were examined by direct electron microscopy (DEM), enzyme-linked immunosorbent assay (ELISA), polyacrylamide gel electrophoresis (PAGE), bacterial culture and biochemical methods Combined with clinical analysis for the first time found that: Kunming area all year round rotavirus gastroenteritis have occurred, the course of acute, persistent and chronic. All row of loose water will be, 25.4% accompanied by mucus will be, part of the course of delay or associated with mucus messenger and mixed infection. There was no difference in clinical manifestations of rotavirus gastroenteritis among different seasons (P> 0.05). The clinical manifestations of gastroenteritis with different electrophoresis rotavirus were also different, suggesting that the toxicity of non-electrophoresis rotavirus was different. Still pointed out that the virus and bacteria, the mixed infection between the characteristics of the virus.