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采用水培实验,研究了溶液酸度、钒(Ⅴ)离子浓度、吸收时间和钒-镉(V-Cd)复合胁迫下枸杞幼苗对V的吸收动力学特性。结果表明,pH是影响枸杞幼苗对V吸收的重要指标,随着溶液pH值增大,枸杞幼苗对V的吸收速率增大;随着吸收时间的增加,枸杞幼苗对V的吸收速率先增大后减小。在pH值为8.5的溶液中培养24 h,枸杞幼苗对V的吸收达到最大值,用Lineweaver-Burk作图法求解得最大吸收速率(Vmax)为1.67×103ng g-1h-1。与单独V处理比较,在V-Cd复合胁迫中,随着Cd(Ⅱ)浓度由0增加到30 mg L-1,枸杞幼苗的Vmax由151.5 ng g-1h-1增加至1.58×103ng g-1h-1,表明Cd(Ⅱ)浓度的增加会刺激枸杞幼苗对V的吸收。
The hydroponic experiment was carried out to study the kinetic characteristics of solution absorption, V (superscript +) concentration, absorption time and V under stress of vanadium-cadmium (V-Cd). The results showed that pH was an important index that affected the absorption of V. By increasing the pH value of the solution, the absorption rate of V to L. lycii L. seedlings increased. With the increase of the absorption time, the absorption rate of V to L. seedlings increased first After the decrease. The maximum absorption rate (Vmax) of Lycium barbarum seedlings reached Vmax when incubated at pH 8.5 for 24 h. The maximum absorption rate (Vmax) calculated by Lineweaver-Burk plot was 1.67 × 103 ng g-1 h-1. Compared with V treatment alone, the Vmax increased from 151.5 ng g-1 h-1 to 1.58 × 103 ng g-1 in the V-Cd stress as Cd (Ⅱ) concentration increased from 0 to 30 mg L-1 1h-1, indicating that the increase of Cd (Ⅱ) concentration will stimulate the absorption of V to the Lycium barbarum seedlings.