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为了明确美洲黑杨锈病的病原菌种类,本研究从江苏南京采集美洲黑杨锈病样品,对病原物形态进行观察,同时进行致病性测定,并对再次致病的病原物进行形态学观察,从江苏南京、江苏泗洪、湖北武汉、湖北石首采集美洲黑杨锈病样品,进行rDNA-ITS序列分析鉴定。结果表明,通过对美洲黑杨锈菌形态特征进行观察,表明长江中下游地区美洲黑杨锈菌的形态与Melampsora larici-populina相似。致病性测定结果表明,所采集锈菌能引起美洲黑杨锈病,与自然发病症状相同,而且发病后的病原物形态特征与自然采集的病原菌相同。根据ITS序列进行聚类,四地的菌株均与M.larici-populina聚在一起,表明长江中下游地区的美洲黑杨锈病病原菌为M.larici-populina。
In order to clarify the pathogen species of Poplar black rust in the Americas, this study collected samples of poplar rust in American poplar from Nanjing, Jiangsu Province. Morphological observation was carried out on the pathogen. At the same time, the pathogenicity was determined and pathogenicity was observed again. Nanjing, Jiangsu Sihong, Hubei Wuhan, Hubei Shishan American black poplar rust samples were collected, rDNA-ITS sequence analysis identified. The results showed that Morphological characteristics of Populus tomentosa were observed in the Americas, which showed that the morphology of Populus tomentosa was similar to that of Melampsora larici-populina in the middle and lower reaches of the Yangtze River. The results of pathogenicity test showed that the collected rust could cause poplar rust, which was the same as the natural disease symptom, and the pathogenic morphological characteristics after the onset were the same as the naturally collected pathogens. According to the ITS sequences clustering, the four strains were clustered together with M.larici-populina, which indicated M.larici-populina was the pathogen of Populus nigra in the middle and lower reaches of the Yangtze River.