寄生隐丛赤壳菌Cryphonectria parasitica菌株经液体培养,石油醚萃取其发酵液获得对板栗带叶嫩枝具有致萎活性的粗提物,以氯仿:石油醚:甲醇(6:2:2)作洗脱剂,粗提物经硅胶色谱分离,共得到3组纯组份,其中第1组份(Cp-I)对板栗幼苗致萎活性较高。质谱、核磁共振和红外光谱测定表明Cp-I分子量为278,化学式为C16H22O4。 Parasitism Cryptocaryon variabilis Cryphonectria parasitica strains were cultured in liquid and extracted with petroleum ether to obtain the crude extract with wilting activity on the leaves of the chestnut with chloroform: petroleum ether: methanol (6: 2: 2) The eluent and the crude extract were separated by silica gel chromatography. Three pure fractions were obtained. The first fraction (Cp-I) had higher wilting activity on the chestnut seedlings. Mass spectrometry, nuclear magnetic resonance and infrared spectroscopy showed that Cp-I had a molecular weight of 278 and a chemical formula of C16H22O4.