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目的应用基因芯片技术筛选人生长激素腺瘤发生、发展相关基因,为后续研究奠定基础。方法应用人全基因组寡核苷酸芯片HG-U133 Plus2.0检测8例生长激素腺瘤组织、2例混合正常垂体组织的基因表达谱,差异表达基因进行筛选和生物信息学分析。Real-time qPCR验证芯片结果。结果筛选出与生长激素腺瘤相关的上调基因187条,下调基因899条;差异表达基因功能主要涉及分子结合、凋亡或肿瘤相关、代谢、信号转导、细胞周期、物质运输等多个生物过程。结论基因芯片技术可初步筛选出人生长激素腺瘤相关基因;生长激素腺瘤的发生、发展是多基因、多分子、多通路的网络调控过程。
Objective To screen human growth hormone adenoma by gene chip technology and to develop related genes, which laid the foundation for further research. Methods Human genome-wide oligonucleotide microarray HG-U133 Plus2.0 was used to detect the gene expression profiles of 8 cases of growth hormone adenoma tissues and 2 cases of normal mixed pituitary tissues. The differentially expressed genes were screened and bioinformatics analysis was performed. Real-time qPCR verification chip results. Results A total of 187 genes were up-regulated and 899 genes were down-regulated in growth hormone adenoma. The function of differentially expressed genes mainly involved in molecular biology, apoptosis, or tumor related, metabolism, signal transduction, cell cycle, process. Conclusion Gene chip technology can screen human growth hormone adeno-associated gene initially. The occurrence and development of growth hormone adenoma are multi-gene, multi-molecule and multi-pathway network regulation process.