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目的探讨藻黄胶囊Ⅲ号(ZHC3)治疗慢性肾功能衰竭的机理。方法采用不同浓度的ZHC3孵育体外培养的人肾间质成纤维细胞(HRIF),观察24h和48h时HRIF增殖与凋亡情况,检测纤维连接蛋白(FN)、层粘连蛋白(LN)、转化生长因子β1(TGF-β1)的分泌水平。结果各浓度的ZHC3在24h和48h时可显著抑制HRIF的增殖(P<0.01);各浓度的ZHC3在24h时减少FN的分泌(P<0.01);25μg/ml和50μg/ml浓度的ZCH3可在48h时减少TGF-β1分泌(P<0.05);对LN的分泌及HRIF的凋亡无明显影响。结论ZHC3可抑制HRIF的增殖,而且可以减少FN、TGF-β1的分泌,这可能是ZHC3治疗慢性肾功能衰竭的机理。
Objective To explore the mechanism of treatment of chronic renal failure with Zhahuang capsule III (ZHC3). Methods Human renal interstitial fibroblasts (HRIF) cultured in vitro with different concentrations of ZHC3 were used to observe the proliferation and apoptosis of HRIF at 24h and 48h. Fibronectin (FN), laminin (LN) and transforming growth were detected. Secretion level of factor β1 (TGF-β1). Results The concentration of ZHC3 significantly inhibited the proliferation of HRIF at 24 h and 48 h (P<0.01). The concentrations of ZHC3 reduced the secretion of FN at 24 h (P<0.01); ZCH3 at concentrations of 25 μg/ml and 50 μg/ml were available. The TGF-β1 secretion was decreased at 48 h (P<0.05); there was no significant effect on the secretion of LN and apoptosis of HRIF. Conclusion ZHC3 can inhibit the proliferation of HRIF and reduce the secretion of FN and TGF-β1. This may be the mechanism of ZHC3 in treating chronic renal failure.