论文部分内容阅读
目的:探讨青蒿琥酯对大鼠蛛网膜下腔出血后早期脑损伤的保护作用。方法:60只大鼠被随机分成对照组、模型组和治疗组,采用颅内动脉血管内穿刺法建立蛛网膜下腔出血模型(对照组为假手术处理)。造模1小时后,对照组和模型组大鼠给予腹腔注射生理盐水;治疗组大鼠接受腹腔注射青蒿琥酯治疗。分别评价三组大鼠神经功能损伤程度、脑水肿情况、血脑屏障(BBB)通透性以及内皮细胞紧密连接蛋白Occludin和ZO-1的表达水平。结果:与模型组相比,青蒿琥酯治疗显著提高了大鼠的神经功能评分,降低了大鼠脑水肿指数和BBB通透性,缓解了Occludin和ZO-1蛋白的表达下调。结论:青蒿琥酯减轻SAH后模型大鼠早期脑损伤的效果迅速而确切,机制可能与调节内皮细胞紧密连接蛋白Occludin和ZO-1的表达有关。
Objective: To investigate the protective effect of artesunate on early brain injury after subarachnoid hemorrhage in rats. Methods: Sixty rats were randomly divided into control group, model group and treatment group. Subarachnoid hemorrhage model was established by intracranial arterial puncture (control group sham operation). One hour after modeling, the control group and model group rats were given intraperitoneal injection of saline; the treatment group received intraperitoneal injection of artesunate. The degree of neurological injury, cerebral edema, BBB permeability and the expression of tight junction proteins Occludin and ZO-1 in the three groups were evaluated respectively. Results: Compared with model group, artesunate treatment significantly increased neurological score, reduced brain edema index and BBB permeability, and alleviated the down-regulation of Occludin and ZO-1 protein expression. CONCLUSION: Artesunate can relieve the early brain injury in SA rats rapidly and accurately, which may be related to the regulation of the expression of Occludin and ZO-1.