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目的:探讨小檗碱对腹腔暴露后大鼠肠黏膜屏障的影响。方法:将大鼠剖腹后暴露腹腔,但不进行任何手术操作,暴露3 h后关腹,制作腹腔暴露模型。将30只大鼠随机分为五组,即对照组、腹腔暴露组、小檗碱低剂量(100 mg/kg)干预组、中剂量(150 mg/kg)干预组和高剂量(200 mg/kg)干预组,每组6只。小檗碱低、中、高剂量干预组分别于术前3 d和术后6 h每天给予一次相应剂量的小檗碱灌胃。对照组和腹腔暴露组在相同条件下分别给予等量的等渗盐水灌胃。术后24 h取血检测血清D-乳酸水平,并取末端回肠组织测定肠道FD4清除率,同时观察末端回肠组织的病理变化,并进行肠黏膜损伤Chiu’s评分。结果:与对照组比,腹腔暴露组大鼠血清D-乳酸水平明显上升(P<0.05),肠道FD4清除率和肠黏膜损伤Chiu’s评分明显增加(P<0.05)。与腹腔暴露组比,各小檗碱干预组大鼠血清D-乳酸水平有所下降,肠道FD4清除率和肠黏膜损伤Chiu’s评分有所降低,以中、高剂量组最为显著(P<0.05)。结论:小檗碱可减轻腹腔暴露引起的肠黏膜损伤,对维持肠黏膜屏障功能具有一定的保护作用。
Objective: To investigate the effect of berberine on intestinal mucosal barrier in rats after intraperitoneal exposure. Methods: The abdominal cavity of rats was exposed after cesarean section, but without any surgical operation, the abdominal cavity was exposed 3 hours after exposure. Thirty rats were randomly divided into five groups: control group, intraperitoneal exposure group, berberine low dose (100 mg / kg) intervention group, middle dose (150 mg / kg) intervention group and high dose (200 mg / kg) intervention group, 6 rats in each group. Berberine low, medium and high dose intervention group were given at the appropriate dose of berberine gavage 3 days before surgery and 6 h after surgery. The control group and intraperitoneal exposure group were given the same amount of isotonic saline under the same conditions. Serum levels of D-lactate were measured at 24 h after operation, and the clearance rate of FD4 in the distal ileum was measured. The pathological changes of the terminal ileum were observed, and Chiu’s score of intestinal mucosal injury was also observed. Results: Compared with the control group, the level of serum D-lactate increased significantly (P <0.05), the rate of intestinal FD4 clearance and the intestinal mucosal lesion Chiu’s score increased significantly (P <0.05). Compared with the intraperitoneal exposure group, the levels of serum D-lactate in the berberine intervention group decreased, the clearance rate of intestinal FD4 and the Chiu’s score of intestinal mucosal injury decreased, especially in the medium and high dose groups (P <0.05 ). Conclusion: Berberine can relieve intestinal mucosal injury caused by peritoneal exposure and protect the intestinal mucosal barrier function.