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目的对高效液相色谱法测定酸枣仁中黄曲霉毒素B1残留量的不确定度进行评定。方法对HPLC测定酸枣仁中黄曲霉毒素B1残留量的全过程进行分析,同时结合酸枣仁中黄曲霉毒素B1的HPLC方法验证数据,对分析过程中称量、供试品溶液及对照品溶液制备等影响因素引起的不确定度进行评价,最后根据各分量计算出合成不确定度并进行了扩展。结果酸枣仁中黄曲霉毒素残留量B1为6.218μg.kg-1,置信概率(P)为95%时,其扩展不确定度为0.496μg.kg-1。结论该方法适用于HPLC测定酸枣仁中黄曲霉毒素B1残留量的不确定度评定,为黄曲霉毒素分析过程的不确定度评定提供了重要参考。
OBJECTIVE To evaluate the uncertainty of determination of aflatoxin B1 residues in wild jujube by high performance liquid chromatography. Methods The whole process of determination of aflatoxin B1 in Semen Ziziphi spinosa L. was analyzed by HPLC. The data of the method was validated by the HPLC method of aflatoxin B1 in Semen Ziziphi spinos L., and weighed in the course of the analysis for the preparation of the test solution and the reference solution And other factors to assess the impact of uncertainty, and finally calculated based on the components of the synthesis of uncertainty and expanded. Results The residue aflatoxin B1 was 6.218μg.kg-1 in Suanzaoren, and its extended uncertainty was 0.496μg.kg-1 when the confidence probability (P) was 95%. Conclusion The method is suitable for the determination of the uncertainty of aflatoxin B1 residues in the wild jujube by HPLC, which provides an important reference for the evaluation of the uncertainty of aflatoxin analysis.