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目的研究雷公藤甲素对雄性性成熟期大鼠附睾功能及精子运动的影响,探讨雷公藤甲素生殖毒性的作用机制。方法将2月龄健康清洁级Sprague-Dawley雄性大鼠40只随机分为对照组(0.5%的羧甲基纤维素钠)及低、中、高雷公藤甲素染毒组(染毒剂量分别为25、50、100μg/kg),经口灌胃染毒,每天1次。连续染毒50d后,取双侧附睾称重,并计算附睾系数。唾液酸含量的测定采用5-甲基苯二酚法,蛋白含量的测定采用考马斯亮兰法,肉毒碱含量的测定采用酶联免疫吸附法。采用计算机辅助精子分析(CASA)系统评价左附睾尾精子动力学参数。结果各染毒组大鼠体重间比较,差异无统计学意义(P>0.05)。与对照组相比,中、高剂量组唾液酸含量和高剂量组附睾脏器系数和肉毒碱含量均较低,差异有统计学意义(P<0.05或P<0.01)。与对照组比较,高剂量组精子曲线速度(VCL)、平均路径速度(VAP)、侧摆幅度(ALH)、鞭打频率(BCF)和中、高剂量组精子直线速度(VSL)、直线性(LIN)、前向性(STR)均较低,差异有统计学意义(P<0.05或P<0.01)。结论雷公藤甲素可以引起附睾功能损伤,继而影响附睾尾精子运动速度与线性方向。
Objective To study the effects of triptolide on epididymal function and sperm motility in male sexual maturation rats and to explore the mechanism of action of triptolide on reproductive toxicity. Methods Forty Sprague-Dawley male Sprague-Dawley rats aged 2 months were randomly divided into control group (0.5% carboxymethylcellulose sodium) and low-, medium-, and high-trehalline exposure groups (toxic dose For 25, 50, and 100 μg/kg, oral gavage once a day. After 50 days of continuous exposure, both sides of the epididymis were weighed and the coefficient of epididymis was calculated. The determination of sialic acid content was performed using the 5-methylbenzenediol method, protein content was measured using the Coomassie brilliant blue method, and carnitine content was measured by enzyme-linked immunosorbent assay. The computer-assisted sperm analysis (CASA) system was used to evaluate the left epididymal sperm motility parameters. Results There was no significant difference in body weight between rats in each group (P>0.05). Compared with the control group, the sialic acid content in the high-dose group and the organ coefficient and carnitine content in the high-dose group were lower, the difference was statistically significant (P<0.05 or P<0.01). Compared with the control group, sperm curve velocity (VCL), mean path velocity (VAP), amplitude (ALH), frequency of whiplash (BCF), sperm linear velocity (VSL), and linearity in the high-dose group (high dose group). Both LIN and STR were significantly lower (P<0.05 or P<0.01). Conclusion Triptolide can cause epididymal function damage, and then affect epididymal tail sperm movement speed and linear direction.