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本文建立了PCR－RFLP和16srDNA指纹图法．对19株幽门螺杆菌（HP）进行基因型分析：HP尿素酶C基因的PCR扩增产物．分别用HindⅢ、HaeⅢ、AluⅠ酶切，结果显示：每个酶均将19株HP分为3种RFLP图谱．综合HindⅢ，HaeⅢ和AluⅠ酶切结果，19株HP分为10个酶切带型；PCR扩增HP标准株16SrRNA基因，地高辛标记制备550bp探针，19株HPDNA分别经HaeⅢ和EcoRⅠ酶切、电泳后，通过Southern杂交获16SrDNA指纹图，结果显示：HaeⅢ酶切分为14个杂交带型，EcoRⅠ酶切19株HP杂交带型均不同。本实验表明：上述两种方法重复性好，分群力高，可准确有效地对HP作出鉴定并将其分型。19株HP株间存在基因型差异。
This article established PCR-RFLP and 16srDNA fingerprinting method. Nineteen H. pylori (HP) genotypes were analyzed: PCR products of the HP urease C gene. Respectively with Hind Ⅲ, Hae Ⅲ, Alu Ⅰ digestion, the results showed that: Each enzyme will be 19 strains of HP is divided into three kinds of RFLP patterns. According to the results of HindⅢ, HaeⅢand AluⅠ digestion, 19 strains of HP were divided into 10 digestion bands; 16SrRNA gene of HP standard strain was amplified by PCR, 550bp probe was prepared by digoxin labeling, 19 HPDNA were digested by HaeⅢ and EcoRⅠ After electrophoresis, 16S rDNA fingerprinting was obtained by Southern blotting. The results showed that Hae Ⅲ digestion was divided into 14 hybridization bands, and the EcoRⅠ digestion of 19 HP bands was different. This experiment shows that: The above two methods have good repeatability and high grouping power, which can accurately and effectively identify and classify HP. There were genotype differences among 19 HP strains.