论文部分内容阅读
建立70%乙醇灌胃致大鼠胃粘膜急性损伤模型,以胃粘膜血流量、胃跨壁电位差、胃粘膜损伤指数为指标,并测定血液中、胃粘膜中一氧化氮(NO)含量,研究针灸对胃粘膜损伤的保护作用及其与NO的关系。结果显示:针灸对胃粘膜损伤具有保护作用。NO的前体L精氨酸(Larg)和NO的供体硝普钠(SNP)对胃粘膜也有保护作用,而NO合成酶的抑制剂N亚硝基左旋精氨酸(LNNA)未显示对胃粘膜有保护作用。Larg和SNP预处理后,可加强针灸对胃粘膜的保护作用,而LNNA则有减弱针灸对胃粘膜的保护作用,用LNNA及Larg同时预处理,则又增强了针灸对胃粘膜保护作用。结论:针灸对胃粘膜的保护作用可能通过LargNO通路实现的。
Acute gastric mucosal injury induced by 70% ethanol was established in rats. Gastric mucosal blood flow, gastric transmural potential difference and gastric mucosal lesion index were used as indexes, and the levels of nitric oxide (NO) in blood and gastric mucosa were determined. To study the protective effect of acupuncture and moxibustion on gastric mucosal injury and its relationship with NO. The results showed that acupuncture had a protective effect on gastric mucosal injury. NO precursors L arginine (L arg) and NO donor sodium nitroprusside (SNP) also have a protective effect on gastric mucosa, and NO synthase inhibitor N nitroso L-arginine (L NNA) did not show a protective effect on gastric mucosa. L arg and SNP pretreatment, can enhance the protective effect of acupuncture on gastric mucosa, while L NNA has reduced the protective effect of acupuncture on gastric mucosa, L NNA and L arg simultaneously pretreatment, then enhanced Acupuncture on gastric mucosal protective effect. Conclusion: The protective effect of acupuncture on gastric mucosa may be achieved through the L arg NO pathway.