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高等植物中甜菜碱由胆碱经两步氧化反应合成,其中由胆碱单加氧酶(CMO)催化第一步氧化反应,甜菜碱醛脱氢酶(BADH)催化第二步氧化反应。采用生物信息学技术和RT-PCR的方法从水稻幼苗组织中分离得到了水稻CMO基因的cDNA克隆,将其命名为OsCMO。该基因含有10个外显子和9个内含子,其开放阅读框编码一条长为410个氨基酸残基的多肽,与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50%~62%。mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达,表明该基因可能在抵御非生物胁迫中发挥重要作用。
Betaine from higher plants was synthesized by two steps of oxidation of choline. The first oxidation was catalyzed by choline monooxygenase (CMO) and the second oxidation by betaine aldehyde dehydrogenase (BADH). The cDNA clone of rice CMO gene was isolated from rice seedling tissue by bioinformatics and RT-PCR and named as OsCMO. The gene contains 10 exons and 9 introns. Its open reading frame encodes a polypeptide of 410 amino acid residues, which is complementary to the choline of Arabidopsis, mountain spinach, amaranth, Suaeda and sugar beet Monooxygenase amino acid sequence identity 50% ~ 62%. mRNA analysis showed that the expression of OsCMO gene in rice seedling tissue was induced by the up-regulation of high salt, low temperature and drought stress, which indicated that OsCMO gene may play an important role in abiotic stress suppression.