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目的：探讨大鼠细小动脉平滑肌细胞分离培养的新方法。方法：取大鼠肺分支动脉，先切成小块进行胶原酶的消化，约 ８ ｈ，而后用含２０％小牛血清的 ＤＥＭＥ培养基贴块培养。结果：培养 ２４ ｈ，可见有大量细胞游出贴瓶底生长，７２ｈ已融合成片，呈典型的“谷峰”样长势。尚有少量内皮细胞，通过消化传代除去。取传第三代细胞进行抗α－ａｃｔｉｎ免疫组织化学染色，大量饮泡，基膜下有密斑，密体。免疫组化鉴定培养细胞纯度为９６％。结论：应用消化贴块法培养的大鼠平滑肌细胞，方法简单，结果可靠，具有应用价值。
Objective: To explore a new method for the isolation and culture of rat arteriolar smooth muscle cells. METHODS: The rat pulmonary artery was dissected and cut into small pieces for digestion of collagenase for about 8 hours. Then, it was cultured in DEME medium containing 20% calf serum. Results: After culturing for 24 hours, a large number of cells could be seen sticking to the bottom of the bottles for growth, and 72 hours after being fused into tablets, showing a typical “Gufeng” -like growth. There are still a small amount of endothelial cells, removed by digestion and passage. Take the third generation of cells for anti-α-actin immunohistochemical staining, a large number of bubble, under the basement membrane with dense spots, dense body. Immunohistochemical identification of cultured cells was 96% purity. Conclusion: The rat smooth muscle cells cultured by digestion and sticking method are simple, reliable, and have application value.