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目的:观察黄芪皂甙和丹参酮ⅡA注射液对大鼠骨骼肌钝挫伤后修复过程中氧化应激的影响。方法:120只雄性SD大鼠随机分为黄芪皂甙组、丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组、生理盐水对照组,每组24只。采用打击装置造成右侧腓肠肌中段钝挫伤模型,分别于损伤局部注射黄芪皂甙、丹参酮ⅡA注射液、黄芪皂甙+丹参酮ⅡA注射液(黄芪皂甙0.5 ml+丹参酮ⅡA注射液0.5 ml混合)、黄芪丹参注射液(黄芪注射液0.5 ml+丹参注射液0.5 ml混合)及生理盐水,注射剂量均为1 ml/只/次,以后每隔3天注射一次,并于损伤后1、4、7和14天取腓肠肌测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-px)活性。结果:与生理盐水对照组相比,丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组MDA含量在第4天时显著降低(P<0.05),而黄芪皂甙组下降不显著;与生理盐水对照组相比,黄芪皂甙组、丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组SOD、GSH-px活性在第4天时显著升高(P<0.05),黄芪皂甙组升高幅度低于丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组,与黄芪丹参注射液组相比,丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组SOD、GSH-px活性无显著差异(P>0.05)。结论:黄芪皂甙和丹参酮ⅡA注射液均可减轻骨骼肌钝挫伤后氧化应激的损伤,并提高骨骼肌SOD和GSH-px的活性,而丹参酮ⅡA注射液作用更为显著。
Objective: To observe the effect of astragaloside and tanshinone ⅡA injection on the oxidative stress in rat skeletal muscle after contusion repair. Methods: One hundred and twenty male Sprague Dawley rats were randomly divided into four groups: Astragalus saponin group, Tanshinone ⅡA injection group, Astragalus saponin + Tanshinone ⅡA injection group, Astragalus Salviae miltiorrhiza injection group and saline control group. The model of blunt contusion in the middle of the gastrocnemius muscle was created by using a striking device. Astragalus saponin, tanshinone ⅡA injection, astragaloside + tanshinone ⅡA injection (0.5 ml + tanshinone ⅡA injection) (Astragalus injection 0.5 ml + Salvia miltiorrhiza injection 0.5 ml mixed) and saline, the injection dose was 1 ml / only / times, after every 3 days after injection, and 1, 4, 7 and 14 days after injury gastrocnemius The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were measured. Results: Compared with saline control group, the content of MDA in tanshinone ⅡA injection group, astragaloside + tanshinone ⅡA injection group and Astragalus Danshen Injection group were significantly decreased on the 4th day (P <0.05), while those in the astragaloside group decreased The activities of SOD and GSH-px in astragaloside group, tanshinone ⅡA injection group, astragaloside + tanshinone ⅡA injection group and Astragalus Salviae Miltiorrhizae injection group were significantly higher than those in control group (P < 0.05). The increase of astragaloside group was lower than that of tanshinone ⅡA injection group, astragaloside + tanshinone ⅡA injection group and astragalus salvia miltiorrhiza injection group. Compared with Astragalus Salviae miltiorrhiza injection group, tanshinone ⅡA injection group, astragaloside + Tanshinone Ⅱ A injection group SOD, GSH-px activity was no significant difference (P> 0.05). Conclusion: Both astragaloside and tanshinone Ⅱ A can relieve oxidative stress injury and increase the activity of SOD and GSH-px in skeletal muscle after blunt traumatic contusion, and the effect of tanshinone ⅡA injection is more significant.