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以紫花亚菊茎段为外植体对其进行组织培养,MS为基本培养基,设置不同激素浓度配比,对试验结果进行观察分析,筛选出适合的配方:启动培养基为MS+6-BA0.5mg·L-1+NAA0.01mg·L-1;继代培养基MS+6-BA0.3mg·L-1+NAA0.05mg·L-1,组培苗分化率高;不定根最适诱导培养基为:1/2MS+IBA0.15mg·L-1,生根率达90%以上,组培苗移栽成活率达95%。
The stem segments of A. violaceum were used as explants for tissue culture. MS was used as the basic medium, different hormone concentrations were set, and the test results were observed and analyzed, and the suitable formula was screened: the start-up medium was MS + 6- BA0.5mg · L-1 + NAA0.01mg · L-1; the subculture medium MS + 6-BA0.3mg · L-1 + NAA0.05mg · L-1, The induction medium was 1 / 2MS + IBA 0.15 mg · L -1, the rooting rate reached more than 90%, and the survival rate of transplanting of tissue culture seedlings was 95%.