论文部分内容阅读
目的：了解慢性排斥患者移植肾中的肥大细胞是否存在不同亚类。方法：采用甲苯胺蓝特殊染色及肥大细胞特异酶Ｔｒｙｐｔａｓｅ（类胰蛋白酶）免疫组织化学染色，对１０例切除的慢性排斥患者移植肾标本进行了肥大细胞形态观察。结果：发现慢性排斥患者移植肾中存在三种类型的肥大细胞。分别为甲苯胺蓝染色阳性，Ｔｒｙｐｔａｓｅ阳性及以上二种方法双阳性的细胞类型。三种类型肥大细胞占肥大细胞总数的百分比分别为：２３１２％；８０５％与６８８６％。结论：经由甲苯胺蓝特染及免疫组化染色证明：在慢性排斥患者移植肾中存在三种不同类型的肥大细胞，其功能特性尚需进一步研究。
OBJECTIVE: To investigate the existence of different subsets of mast cells in renal transplant recipients with chronic rejection. Methods: Mast cell morphology was observed in 10 cases of excised chronic rejection patients by Toluidine blue staining and tryptase immunohistochemical staining. Results: Three types of mast cells were found in the renal transplant recipients with chronic rejection. Respectively, toluidine blue staining, Tryptase positive and the above two methods double positive cell type. The percentages of the three types of mast cells in the total number of mast cells were 23.12%, 805% and 6886% respectively. Conclusion: Toluidine blue staining and immunohistochemical staining demonstrated that there are three different types of mast cells in the renal transplant recipients with chronic rejection, and their functional properties need further study.