论文部分内容阅读
采用计算机辅助分析和电子显微镜方法,对大菱鲆(Scophthalmus maximus)精子生理活性(运动率、运动速度和寿命)和超微结构进行观察,研究其精子经超低温保存后质量变化情况,得到了新鲜精子的路径速度[(101.91±9.30)m/s]、运动率(88.30%±2.62%)和寿命[(368.00±111.50)h],以及冷冻精子的路径速度[(76.78±8.49)m/s]、运动率(65.60%±4.76%)和寿命[(120.00±12.00)h]。结果表明,新鲜精子的生理特性好于冷冻精子。同时通过电镜去进行超微结构分析,发现大菱鲆精子经过超低温保存后,40%—60%的精子基本保持正常的形态结构,其余精子遭受不同程度的机械损伤。其中膜损伤为主要损伤,损伤精子中60%—70%带有膜损伤。推测大菱鲆精子在冷冻解冻过程中遭受到的机械损伤可能是导致冻精生理活性显著下降的主要原因。
The physiological activities (motor rate, exercise speed and life span) and ultrastructure of Scophthalmus maximus were observed by computer-aided analysis and electron microscopy. The quality changes of spermatozoa after cryopreservation were studied. Fresh Sperm path velocity [(101.91 ± 9.30) m / s], exercise rate (88.30% ± 2.62%) and longevity [(368.00 ± 111.50) h], as well as spermatic path velocity [(76.78 ± 8.49) m / s ], Exercise rate (65.60% ± 4.76%) and life expectancy [(120.00 ± 12.00) h]. The results showed that fresh sperm physiological characteristics better than frozen sperm. At the same time through the electron microscopy to carry out ultrastructure analysis found that turbot turbid sperm after cryopreservation, 40% -60% of the sperm basic normal morphological structure, the rest of the sperm suffer different degrees of mechanical damage. Among them, the membrane damage is the main injury, and 60% -70% of the injured sperm have membrane damage. It is speculated that the mechanical damage suffered by turbot spermatozoa during freezing and thawing may be the main reason leading to the significant decrease of physiological activity of frozen sperm.