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目的:研究微RNA（miRNA-7）在pSS B细胞中的表达，分析其和磷酸酶与张力蛋白同源物（PETN）、疾病活动度的关系。方法:收集2017—2019年青海省人民医院门诊及住院部收集到的20例初发pSS患者为病例组，20名体检健康志愿者作为对照组，收集病例组疾病活动相关实验室检查。运用实时荧光定量反转录聚合酶链反应（RT-qPCR）检测2组B细胞中miRNA-7和PETN mRNA的表达量，分析病例组血浆及B细胞中miRNA-7表达量的一致性。蛋白质印迹法检测2组B细胞中PETN蛋白的表达，分析病例组B细胞中miRNA-7的表达与疾病活动度的相关性，线性回归分析miRNA-7和PETN mRNA之间的关系。结果:和对照组miRNA-7（0.39±0.11）、PTEN mRNA（2.32±0.30）和蛋白（1.03±0.21）比较，病例组B细胞中的miRNA-7（0.53±0.17）表达增高，PTEN mRNA（0.88±0.24）和蛋白（0.51±0.12）表达均降低，差异均有统计学意义（n t=2.990，n P<0.05；n t=16.98，n P<0.05；n t=8.41，n P<0.05）。线性回归分析显示PTEN mRNA与miRNA-7呈负相关（n b=-0.78，n P<0.01），病例组miRNA-7的表达与ESSDAI、IgG、IgA和抗SSB抗体呈正相关，与C4和白细胞呈负相关。n 结论:miRNA-7和疾病活动之间有一定的关系，在B细胞中miRNA-7可能通过PETN的调控参与pSS的发病机制，对疾病活动度评估具有一定价值。“,”Objective:To study the expression of miRNA-7 in B lymphocytes of primary Sj?gren's syndrome (pSS) and its relationship with phosphatase andtensin homolog deleted (PETN) and disease activity.Methods:Twenty newly diagnosed outpatient and inpatient pSS patients were used as case group collected from January 2017 to December 2019 of Qinghai Provincial People's Hospital. Twentyhealthy persons were used as the control group. Disease-related indicators of the case group were collected. Quantitative polymerase chain reaction (RT-qPCR)was used to detect miRNA-7 and PETN mRNA expression in B lymphocytes of the two groups and the consistency between miRNA-7 expression in the plasma and B lymphocytes of the case group was analyzed. Western Blotting method was used to detect the PETN protein in B lymphocytes of the two groups. Correlation analysis was used to analyze the relationship between miRNA-7 expression in B lymphocytes and disease activity in the case group. Linear regression analysis was performed between miRNA-7 and PETN mRNA.Results:The expression of miRNA-7 (0.53±0.17) in the B cells increased and the expression of PTEN mRNA (0.88± 0.24) and protein (0.51±0.12) in the case group were reduced compared with that of miRNA-7(0.39±0.11), PTEN mRNA(2.32±0.30) and protein(1.03±0.21) of the control group. The above differences were statistically significant (n t=2.990,n P<0.05;n t=16.98,n P<0.05;n t=8.41,n P<0.05). Linear regression showedthat PTEN miRNAwas negatively correlated with miRNA-7 (n b=-0.78, n P<0.01), the expression of miRNA-7 in the case group was positively related with EULAR Sj?gren′s syndrome Disease Activity Index (ESSDAI), IgG, IgA, anti-SSB and was negatively correlated with C4 and WBC.n Conclusion:There is a certain relationship between miRNA-7 and disease activity. MiRNA-7 may participate in the pathogenesis of pSS byregulating PETN in B cells of pSS. MiRNA-7 has certain clinical value for disease activity evaluation.