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目的探讨紫金标抗单纯疱疹Ⅰ型病毒(HSV-1)的分子机制,为进一步开发该药提供理论依据。方法采用不同剂量的紫金标作用于适量HSV-1感染的Vero细胞,以50%组织细胞感染量(TCID50),细胞病变效应(CPE),蚀斑形成单位(PFU)为评价指标,采用CsCl密度梯度离心,RT-PCR,Western Blot以及扫描电镜和透射电镜为研究手段。结果紫金标无直接灭活HSV- 1的作用,也不能影响病毒的释放;但可干扰HSV-1对宿主细胞的吸附,选择性地抑制HSV-1 DNA及蛋白质合成,不同浓度的紫金标能明显抑制HSV-lgD基因mRNA表达。结论紫金标能抑制HSV-1对宿主细胞的吸附选择性地抑制HSV-1 DNA及蛋白质合成,抑制HSV-1gl)基因转录
Objective To investigate the molecular mechanism of Zijin anti-herpes simplex virus type 1 (HSV-1) and provide theoretical basis for further development of the drug. Methods Different doses of Zijin were used to treat the HSV-1-infected Vero cells with 50% tissue-tissue infection (TCID50), cytopathic effect (CPE), and plaque formation unit (PFU) as evaluation indicators, and CsCl density was used. Gradient centrifugation, RT-PCR, Western Blot, and scanning electron microscopy and transmission electron microscopy were used as research methods. RESULTS: Zijin did not directly inactivate HSV-1, nor could it affect the release of virus; however, it could interfere with HSV-1’s adsorption to host cells and selectively inhibit HSV-1 DNA and protein synthesis. Different concentrations of Zijin could The HSV-lgD gene mRNA expression was significantly inhibited. Conclusion Zijin can inhibit the adsorption of HSV-1 to host cells and selectively inhibit the synthesis of HSV-1 DNA and protein and inhibit the transcription of HSV-1 gene.