目的体外制备红细胞的方法大多使用脐血、骨髓或外周动员的CD34+细胞或胚胎干细胞作为起始材料,本研究拟利用外周血单个核细胞为原料在体外生产成熟红细胞。方法以血液滤白后剩余的白膜层为原料,从中分离出单个核细胞,然后通过四步培养制备出成熟红细胞。结果经过不同细胞因子 Objective To prepare erythrocytes in vitro mostly using cord blood, bone marrow or peripherally mobilized CD34 + cells or embryonic stem cells as starting materials. In this study, we plan to use peripheral blood mononuclear cells as raw materials to produce mature erythrocytes in vitro. Methods Monolayer cells were separated from the remaining white membrane after blood filtration, and then mature erythrocytes were prepared through four-step culture. Results through different cytokines