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目的:建立克痹骨泰胶囊中血竭素的高效液相色谱含量测定方法。方法:采用Shim-packVP-ODSC18(250mm×4.6mm,5μm)色谱柱;流动相为乙腈-0.05mol.L-1磷酸二氢钠溶液(40∶60);流速1.0mL·min-1,柱温25℃,检测波长440nm。结果:血竭素在16.32～81.60mg·mL-1范围内,呈良好的线性关系,回归方程为Y=14535.1X+16617.9,r=0.9999,平均回收率为97.22%,RSD为1.04%。结论:本方法灵敏度高,准确,重复性好,可作为克痹骨泰胶囊中血竭素含量测定方法。
OBJECTIVE: To establish a method for the determination of Dracorhodin in Kejiaogutai Capsules by high performance liquid chromatography. Method: A Shim-pack VP-ODSC18 (250mm×4.6mm, 5μm) column was used. The mobile phase was acetonitrile-0.05mol.L-1 sodium dihydrogen phosphate solution (40∶60); flow rate was 1.0mL·min-1. Temperature 25 °C, detection wavelength 440nm. RESULTS: In the range of 16.32-81.60 mg·mL-1, Draucin exhibited a good linear relationship. The regression equation was Y=14535.1X+16617.9, r=0.9999. The average recovery was 97.22% and the RSD was 1.04%. Conclusion: This method has high sensitivity, accuracy and good repeatability. It can be used as a method for the determination of dang calliin in Kejiaogutai Capsule.