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随着物理学和生物化学等学科的发展,各学科间的相互渗透以及各种新方法、新手段的涌现,使细菌分类的研究又从细菌的形态、染色反应、酶反应、生理生化特征、营养需求等发展到应用亚细胞结构,如细胞壁组分、蛋白质组分的凝胶电泳等。近年来又应用分子生物学中核酸研究和遗传学技术于分类学,如基因组的 G+C%含量测定,DNA-DNA 分子杂交,以及 rRNA 的寡核苷酸序列分析等,这些结果使我们有可能从多方面观察细菌,更深化了细菌分类学的研究。耶尔森氏菌属的确立和发展就是极为明显的例证。
With the development of disciplines such as physics and biochemistry, the interpenetration of various disciplines and the emergence of new methods and new methods have led to the researches on bacterial taxonomy from the aspects of bacteria morphology, dyeing reaction, enzyme reaction, physiological and biochemical characteristics, nutrition Demand and other applications to the development of subcellular structures, such as cell wall components, protein gel electrophoresis. In recent years, the application of nucleic acid research and genetics in molecular biology to taxonomy, such as genomic determination of G + C content, DNA-DNA molecular hybridization, and oligonucleotide sequence analysis of rRNA have led us to Bacteria may be observed in many ways, deepening the study of bacterial taxonomy. The establishment and development of Yersinia is a very obvious example.