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目的 利用动物实验证实白细胞介素 6( IL-6)对未成熟脑的损伤作用 ,探讨 IL -6导致脑损伤的可能机制。 方法 在新生大鼠静脉内及侧脑室内注射不同剂量的重组人 IL-6( rh IL-6) ,分别于注射后 2 4及 72 h后处死 ,观察脑组织的病理变化。 结果 静脉注射 rh IL-61 0 0 0、50 0 0和1 0 0 0 0 U后 2 4 h,新生鼠脑组织表现为血管周围水肿及神经细胞的缺血性变 ,不同剂量 rh IL-6所致病变程度无明显差异。 72 h后血管周围水肿未见消退 ,神经细胞的缺血性变进一步发展为均质性变。侧脑室内注射 rh IL-61 0 0 0 U后 2 4 h脑组织病变与静脉注射后 2 4 h的病理表现基本相同 ;而注射rh IL-650 0 0 U后 2 4 h,蛛网膜下腔出血较静脉注射组明显。侧脑室内注射 72 h后 ,除有上述表现外 ,还出现了局部的脱髓鞘改变。 结论 外源性 IL-6对新生大鼠脑有不同程度的损伤作用。
OBJECTIVE: To investigate the possible mechanism of interleukin-6 (IL-6) injury in immature brain by animal experiments. Methods Different doses of recombinant human IL-6 were injected intravenously and intracerebroventricularly into neonatal rats. The rats were sacrificed at 24 and 72 hours after injection respectively. Pathological changes of brain were observed. Results The brain tissue of neonatal rats showed edema of perivascular and ischemic changes of nerve cells 24 h after intravenous injection of rhIL-6100, 5000 and 100000 U, and different doses of rhIL-6 The degree of disease caused by no significant difference. 72 h after the perivascular edema did not subside, the ischemic nerve cells further developed into a homogeneous change. The intracerebroventricular injection of rhIL-6100 0 U 2 h after 24 h brain pathology and intravenous injection of 24 h after the pathological manifestations of the same; and rh IL-650 0 0 U 24 h, subarachnoid space Bleeding than the intravenous group was significantly. Intracerebroventricular injection of 72 h, in addition to the above performance, but also appeared in local demyelination. Conclusion Exogenous IL-6 can damage the brain of neonatal rats to some extent.