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采用随机扩增多态DNA(RAPD)方法对6个群体30丛撑篙竹个体进行了遗传变异的研究。28个随机引物共检测到173个位点,其中85个是多态位点,平均每个引物提供6 18个RAPD信息量,扩增出的DNA片段大小一般在200～2000bp范围之间;用POPGENE1 31版软件进行遗传多样性分析:平均Nei’s基因多样性为0 2114,Shannon’s信息指数为0 3277,基因分化系数0 1853,表明群体间有一定的分化;各群体平均遗传距离0 0350,表明群体亲缘关系较近;试用UPGMA方法对不同产地的撑篙竹群体作聚类分析,初步可将6个群体聚为3类。
Random amplified polymorphic DNA (RAPD) method was used to study the genetic variation of 30 individuals in 6 populations. A total of 173 random loci were detected by 28 random primers, of which 85 were polymorphic. The average number of RAPD information provided by each primer was 6 18, and the size of amplified DNA fragments was generally in the range of 200-2000 bp. POPGENE1 31 software for genetic diversity analysis: the average Nei’s gene diversity was 0 2114, Shannon’s information index was 0 3277, the coefficient of genetic differentiation 0 1853, indicating that there is a certain differentiation among populations; the average genetic distance of each population 0 0350, indicating that the population The genetic relationship was close. The UPGMA method was used to cluster the stigmata populations of different origins, and the six groups could be grouped into three categories initially.