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A gas-filled time-of-flight (GF-TOF) detector has been built and developed to improve the ability of isobaric identification in accelerator mass spectrometry (AMS) measurements, and a time resolution (without gas filled)of better than 350ps is achieved. The GF-TOF detector is tested by means of measuring a standard AgCl(36Cl/Cl = 7.6 × 10-9g/g) sample with the 36Cl ion energy of 64, 49 and 33MeV, respectively. 36Cl and 36S particles were successfully separated in the TOF spectra output from the GF-TOF detector. The comparison between the gas-filled time-of-flight method and the △E - E method is described. Some results relative to the GF-TOF method are given as well.