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采用PAP SPA-HRP,间接酶标三种免疫组化方法,用国内外四种不同来源的CEA抗血清及CEA单克隆抗体对62例食管癌(50例食管鳞癌,12例食管腺癌)的手术标本,100例食管拉网涂片,57例8—40周龄的胎儿食管组织切片进行CEA定性,定位及半定量的观察,结果表明食管鳞癌CEA阳性率为96％。食管腺癌为75％,食管拉网涂片为42％,高于涂片的病理形态学诊断(29％),二者的符合率为86％,8～40周龄的胎儿食管上皮细胞均呈CEA阳性,以16—17周龄时CEA阳性反应最强
Using PAP SPA-HRP, indirect enzyme labeling with three immunohistochemical methods, using four different sources of CEA antiserum and CEA monoclonal antibodies at home and abroad for 62 cases of esophageal cancer (50 cases of esophageal squamous cell carcinoma, 12 cases of esophageal adenocarcinoma) Surgical specimens, 100 cases of esophageal pull-net smears, 57 cases of 8-40 week-old fetal esophageal tissue sections were subjected to CEA qualitative, localization and semi-quantitative observations. The results showed that the CEA positive rate of esophageal squamous cell carcinoma was 96%. Esophageal adenocarcinoma was 75%, esophageal mesh smear was 42%, higher than the pathological diagnosis of smear (29%), the coincidence rate of the two was 86%, and fetal esophageal epithelial cells were 8 to 40 weeks old. CEA positive, with the strongest CEA positive response at 16-17 weeks of age