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利用5个来自于普通菜豆抗炭疽病基因SCAR标记(SCA1000、SH181100、SAB3400、SB12350和SCF101072)引物组,对143份荚用菜豆资源进行DNA水平的鉴定。同时使用苗期接种方法,验证了143份资源的抗病性。结果表明:共有61份资源检测到SCAR标记。在这些资源中,有40份具有SCA1000标记,40份具有SCF101072标记,19份兼有SCA1000和SCF101072标记,其余的82份未检测到SCAR标记。这143份资源中均未检测到SAB3400、SB12350和SH181100扩增带。群体抗性鉴定结果显示:143份资源中,抗病的有16份。其中含有2个SCAR标记,群体抗性结果表现抗病的有5份,含有1个SCAR标记,并且群体抗性结果表现抗病的有3份。通过本研究明确了:荚用菜豆中Co-2和Co-10基因含量比较丰富,除扁圆荚类型紫荚菜豆中Co-2基因含量多于Co-10基因外,这两个抗病基因在其它类型的菜豆中分布比较均匀。在油豆类型和扁圆荚类型绿荚菜豆资源中,同时含有此两个抗病基因标记的较多。在油豆类型资源中,含有两个抗病标记,并且群体抗性表现抗病的最多。
DNA was identified in 143 pods of Brassica campestris using five primer sets from the common bean anti-anthracnose gene SCAR markers (SCA1000, SH181100, SAB3400, SB12350 and SCF101072). At the same time, seedling inoculation method was used to verify the resistance of 143 resources. The results showed that: a total of 61 resources detected SCAR markers. Of these resources, 40 have SCA1000 markers, 40 have SCF101072 markers, 19 have both SCA1000 and SCF101072 markers, and the remaining 82 have not detected SCAR markers. None of 143 resources detected SAB3400, SB12350 and SH181100 amplification bands. Identification of population resistance showed that among the 143 resources, 16 were disease resistant. Which contains two SCAR markers, the results of the population resistance showed resistance to 5, contain a SCAR marker, and the population resistance results showed resistance to 3 copies. Through the study, it is clear that Co-2 and Co-10 genes in Podocarpus indica are abundant, except for Co-2 gene in Podocarpus violaceus, which is more than Co-10 gene. In other types of beans are more evenly distributed. In the oil bean type and oblate pod type green bean resources, both contain more of the two resistance gene markers. In the oil-bean type resource, there are two resistance markers, and the population resistance shows the most resistance.