论文部分内容阅读
目的:研究姜黄素抗兔动脉粥样硬化(AS)的作用机制。方法:30只雄性日本大耳白家兔,其中给予高脂饲料喂食2个月,复制AS模型家兔20只,随机分成治疗和对照两组,各10只。均予基础饲料喂养,治疗组每天给予姜黄素(200mg/kg)灌胃,对照组每天给予生理盐水灌胃,共治疗1个月。治疗后处死家兔,观察两组主动脉粥样斑块面积比;取主动脉弓做病理切片;免疫组织化学检测主动脉壁主动脉基质金属蛋白酶9(MMP-9)和核转录因子-κB(NF-κB)的表达。结果:治疗组斑块面积/主动脉面积比低于对照组(P<0.05)。免疫组化显示,治疗组MMP-9、NF-κB阳性细胞表达率明显低于对照组,分别为[(16.5±8.0)%vs(38.5±13.6)%,P<0.05;(22.61±7.95)%vs(42.28±19.34)%,P<0.05]。结论:姜黄素可以显著降低动脉粥样硬化家兔主动脉壁MMP-9、NF-κB的表达,抑制动脉粥样硬化的形成。
Objective: To study the mechanism of action of curcumin on rabbit atherosclerosis (AS). METHODS: Thirty male Japanese big-eared white rabbits were treated with high-fat diet for 2 months and 20 AS model rabbits were randomly divided into treatment and control groups with 10 rats in each group. All basal diets were fed. The treatment group was given intragastric administration of curcumin (200 mg/kg) daily, and the control group was given intragastric administration of normal saline every day for a total of 1 month. After treatment, rabbits were sacrificed to observe the aortic plaque area ratio of the two groups; the aortic arch was taken for pathological section; and the aortic wall aortic matrix metalloproteinase-9 (MMP-9) and nuclear transcription factor-κB (NF) were detected by immunohistochemistry. -kappaB) expression. Results: The ratio of plaque area to aortic area in the treatment group was lower than that in the control group (P<0.05). Immunohistochemistry showed that the expression rate of MMP-9 and NF-κB positive cells in the treatment group was significantly lower than that in the control group, which was [(16.5±8.0)% vs (38.5±13.6)%, P<0.05; (22.61±7.95). %vs (42.28±19.34)%, P<0.05]. Conclusion: Curcumin can significantly reduce the expression of MMP-9 and NF-κB in the aortic wall of atherosclerotic rabbits and inhibit the formation of atherosclerosis.